Infrastructure

The program currently has 11 laboratories (approximately 1000 m2) that attend the teaching and research of graduate studies in biotechnology. The laboratories offer technical support that enable the development of the 6 research lines present in the two areas of concentration of the program.  Therefore, the diversity of models in the research group is based on the generation of knowledge in biotechnology in a multidisciplinary way.

This space of 60m² was donated to the program of the PGBiotec course in 2012 and is operating from 2013. Three projects were approved for this laboratory in CAPES and FAPESB. The LABMUSO, as it is known, consists of 3 environments, with benches, aseptic chambers and safety for work with toxic chemicals and microorganisms. It has equipment acquired with resources from CAPES Pro-Equipamentos 2010 such as Thermocyclador VERITI 96 WELL – APPLIED BIOSYSTEMS, Vortex type agitator for tubes 0.5 to 50 Ml (VIXAR BRAND), non-timer orbital agitator table (CIENTEC BRAND), pH-meter 0.0-14.0 (BRAND PHTK), analytical balance 0, 01g Cap 310g (BEL MARK), precision scale, cap 1000g (BEL MARK), electrophoresis source LPS 300V (BRAND LOCCUS), refrigerated microcentrifuge (THERMO BRAND) and those acquired with project resources Proinfra – FAPESB, as a microprocessed greenhouse for culture of microorganisms – Quant. 2, Greenhouse Mini-Incubator, Processed, Horizontal Freezer, 213 L, 1p, 127 V-Qty. 2, domestic refrigerator, 1 port, 127 V-Qty. 2, purification System/Ultra Ir/Uv Water Filtration, Nobreak equipment, Vortex-quant. 2, Orbital agitator with temperature control-Qty. 3, Voltage Stabilizer-Qty. 3, Potentiometer-Qty. 2, ThermoCycler, set for vertical electrophoresis, sets of micropipettes , glassware, reagents and culture media, with electrical installations, hydraulics, Internet network, all operational. This multi-user profile laboratory is managed by an experienced teacher of the permanent faculty, and is used by masters and doctoral candidates (from various advisors) who develop their basic activities of their experiments, besides Learn to deal properly with instruments and equipment. This laboratory also acts as undergraduate students who develop their projects under the supervision of Masters and doctoral candidates.

This laboratory installed and inaugurated in 2012 has countertop, hydraulic network, telephony, Internet and functional electrical and the following equipment acquired through the REUNI: Vertical Freezer-320 L, Brand: GE-Qty 3, Voltage Stabilizer, CAP, Brand SMS, Shaker bench Incubator, SL 222, orbital agitation, laminar flow Chapel for microbiological protection, vertical Autoclave, 20 L, Brand: FANEM, Spectrophotometer SP-22, Biospectrum, digital ultraviolet microprocessed, swimwear Maria Digital, Brand: EDULAB, refrigerators FrostFree, 02 doors, Brand: Consul-Quant. 3, dual block ThermoCycler 96 X 0.2, CO2 incubator with air jacket, UV disinfection, bench freeze dryer, model L 101, temp. Up to 55 º, glass fiber exhaust chapel, acrylic window, melting point determination apparatus, useful warming blanket for up to 1 liter balloons, body insulator, digital bench PHmetro, brand name: Del Lab-Qty 2, Bath Ultrasonic heating, Brand: Cristofoli, medium heat plate, Brand: New technique, microprocessed refrigerated centrifuge, Digital refractometer, Brand: Atago, vacuum pump in cast bronze, Brand: chimis, microwave oven, cap. 20 L, Midea, greenhouse for culture and convection bacteriology 81 L, source of electrophoresis, 300 V.

a) The Immunodiagnostic laboratory that occupies an area of 378 m2, divided into 12 rooms, between laboratories, classroom, teachers ' rooms/researchers, etc. In addition to supporting research projects, this sector carries out an annual routine of about 400,000 serological tests for SUS patients and offers internships and recycling for students and professionals. It has eight automatic immunoassay systems, three of them through conventional ELISA, three using immunoluminescence and two immunofluorimetry. It has solid computer infrastructure, common CPD with powerful server and a network of 16 microcomputers on intranet and connected to the Internet. It also has a cold chamber with 5, 2M2 and several smaller equipments such as readers and washers for ELISA, pH-meters, agitators, analytical and precision scales, centrifuges, refrigerators and freezers;

 

b) The research sector, with 392 m2, comprising three sectors: the Laboratory of immunity Studies in domestic animals, having a freezer at-70 º C, a freeze-dryer, a bacteriological greenhouse a CO2 greenhouse, two laminar flow chapels (one horizontal and another Vertical), a refrigerated bench centrifuge, a 3,600-rpm centrifuge, an optical transillumination microscope, an inverted optical microscope, a system for ELISA (reader and washer), two agitators, a precision scale and another analytical, a pHmetro, two complete systems for gel electrophoresis and a system for Western blotting; The laboratory of Immunity Studies in Human Diseases, possessing a CO2 greenhouse, two laminar flow chapels (one horizontal and one vertical), a refrigerated bench centrifuge, a 3,600-rpm centrifuge, a microcentrifuge, a thermocycler, two Agitators, a precision scale and other analytical, a pHmetro, a complete system for gel electrophoresis, and a system for Western blotting, a UV-visible spectrophotometer and a system for high performance chromatography; The Molecular Genetics Laboratory has a DNA sequencer with eight capillaries, a thermocycler for real-time PCR, three conventional thermocyclers, two photodocumentation systems, a laminar flow chapel, a refrigerated centrifuge, a Flow cytometer, three complete systems for gel electrophoresis, one freezer at-150 º C., two complete optical microscopes (incandescent transilumination, fluorescence, phase contrast and polarization), coupled with software capture system For conventional capture and FISH. Each of these laboratories has a set of two refrigerators and two freezers and about five microcomputers, in addition to those connected to instruments. In common area it has sinks for washing, equipment for sterilization, water purification and ice making machine. Still as a common structure for these laboratories, there is a student room with five microcomputers, a meeting room/lessons with a Datashow and a microcomputer coupled on a 20'' monitor, an air-conditioned room for mice, one for mice,  Another with a sterile chapel for handling these animals and a covered area for meals.

It has a total physical area of 150m², distributed in 9 separate compartments, all with temperature control, where research and extension activities are carried out using conventional and molecular microbiological methods, techniques Chromatographic (ionic), microscopic molecular (FISH), anaerobic and microaerobic cultivation, preservation of biological and genetic resources (freezer and freeze-drying), pre-PCR room, storeroom, power plant. The laboratory, equipped with operational electrical and hydraulic installations, has a biosafety certificate in 0099/99, Level 2, issued by the CTNBio of the MCT (1999). Among these equipments highlight: Distillers cap. 250 L/h, water purification system by reverse osmosis (Milli-Q) Millipore, Biocell infrared Water Filtration system (Millipore), horizontal Autoclave capacity 100 L (BAUMER), horizontal Autoclave capacity 25 L (Phoenix), Multi-incubador ( 2007), Greenhouses in general (3) being 2 Microporocessadas, BOD, Porometer, gas and Ionic chromatographs, Olympus epifluorescence microscope with video camera and software for Cell Count (2005), spectrophotometers, PCR machines, centrifuges, Laminar Flow Enclosures (02) being one with exhaust for testing and weighing of toxic substances, water purification system (Milli-Q), Refrigerated microcentrifuge Savant (1998), EPPENDORF (2006, 2007), Thermoclicadorbiometra (1998); Anaerobiosis system by substitution of Atmosphere (1988), Chromatograph gaseous (1988) and electrochemical chromatograph, fluorescence and electrical conductivity (Dionex ICS 3000) (2005, 2007), X-Ray Spectrophotometer (2010), Thermomix (2001), Sistema Cryopreservation (2001), photodocumentation system MP4 UV/Visible (2001), documentation system in blue Light (2008), Electrophoresis Apparatus (1998), Vacuum pumps 1/3 CV, greenhouse with Innova agitation 4080 (2001), Microincinerators (2001, 2004, 2007) Being two electrical, pHmeterCorning (2001), Desktop Microcomputers (2001.2003, 2005, 2007) and Laptop (2003, 2006, 2008, 2011, 2011), Anaerobiosis Systems (BACTRON IV, 2004 and III, 2007), epMotion (sample preparation Robot-Eppendorff, 2008), Modern Coolers (4) with double glass door (2) and two side-by-side with access hatch, Multispectrophotometric Reader (Victor 3, PE-2005), real-time PCR 7500 (ApplBiosystems, 2005), DGGE System (2008), bioreactors being two glass with Several cells and a double type in steel 2507 (2008) with capacity for 4 liters each, equipped with temperature control systems and monitoring of chloride, pH and Eh. There is another 1-litre Siris reactor with agitation (2010), with a platform for upgrading and scaling. Computer Clusters, pressure conditions, appropriate anaerobiosis and temperature, microscopes, Nikon optical Microscope (1987), Peristaltic Pumps (2005, 2007) and Heater Blocks (2005), Lyophilizer (2007), Freezer at-86 ° C of the form- Thermolectron (2006) with CO2 Backup, Tensiometer for IF and superficial tension measurements, solid sample Homogenizer (2010), ultrasound Cuba, various peristaltic pumps, bench centrifuges (2), Eppendorf 45R Refrigerated Centrifuge (2010), Lyophilizer, Biocell water purification system and Millipore (industrial with capacity to process 250 L. h-1). In 2010, the collection of microorganisms cultures of LABEM (CCMICS) was created, which has about a thousand specimens of strains of bacteria and fungi, with the majority isolated from samples from the petroleum industry. It has several small functional equipments. A pre-PCR room where the samples are prepared. It also has video conferencing system, room for Secretariat, PG students (08), for PhD (02) and teachers (04). Connected to the web via fiber optic cable.

The activities developed include animal cell culture, viral isolation, viral serological diagnosis, production of monoclonal antibodies and molecular biology techniques. The virology laboratory currently consists of two rooms of 62 square meters and another of 40 square meters in which studies are developed in the areas of human and animal virosis. The virology laboratory has the following infrastructure: 1 horizontal laminar flow for viruses, 1 vertical laminar flow for cell cultivation, 2 horizontal laminars type bench for work with DNA/RNA, 1 DNA sequencer, 1 microscope Inverted for cell culture, 1 fluorescence microscope with photo system, 1 light field optical microscope, 1 ELISA reader, 1 vacuum pump/Millipore tank for media filtration, 1 water bath, 2 pH-meters, 1 drying greenhouse, 2 Sterilization, 1 autoclave, 3 thermocyclers for PCR, 2 systems of vertical electrophoresis for SDS-PAGE, 1 electrophoretic transfer system, 2 horizontal electrophoresis systems, 2 Digital scale, 4 Freezers, 4 refrigerators, 1 freezer-70 º C, 2 greenhouses of CO2, 1 HITACHI refrigerated centrifuge, 1 centrifuge for table tubes. 3 Microcentrifuga for Eppendorf tubes, 1 heating blocks, 3 magnetic stirrers, 1 ultraviolet light transiluminator, 1 photo system for Transiluminator, 1 photo system with, transiluminator/digital system, 2 systems Ultra-Pure purification of Milli-Q water, 15 precision micropipettes.

It has a cell culture room with vertical laminar flow, centrifuges, freezer and refrigerator exclusively for this procedure; Freezer-70C; ELISA reader; Freezers, refrigerator.

Vertical laminar flow, glassmaking, CO2 incubators, microbiological incubators, autoclave, freezers, refrigerators, agitators (shakers).

 

Laboratory of Applied Microbiology and bioprospecting

 

It occupies an area of 18m², endowed with two aseptic chambers biological type II, microbiologic greenhouses, optical microscope, fluorescence microscope, centrifuges, freezers, refrigerators, autoclave. Thermocyclers, DGGE System (2013) and several other equipments that are being acquired with the new project financed by PETROBRAS in July 2012, worth about 1.3 million reais.

The Biophotonics Center consists of 5 laboratories conform below:

 

a) Laboratory of Animal Experimentation: it has rooms for performing surgeries under general anesthesia for rodents, maintenance room for rodents, room for sample handling, locker room, medical and storeroom. In addition, we have access to the Bioterians of the HEI, which have animal breeding and maintenance biotgues and infrastructure for clinical and surgical manipulation of small experimental animals. 01 Vacuum Saliva, 01 electronic scale, 01 water purifier apparatus, 60 boxes for animal restraint, 02 Digital timer, 01 Tosar machine, 01Contensor for rabbits, 01 Autoclave, 01 water Distiller, 01 liquid nitrogen cylinder, 01Car for Garbage, 01 transport car, 01 microinsulator for rodents, miscellaneous furniture, PC, printer, various glassware, miscellaneous surgical instruments. Wireless network.

 

b) Laboratory of Photomicrography Anton van Leeuwenhoek: Axiostar Plus Trinocular microscope with transmitted illumination for light field and dark field, Carlzeiss brand, 2-Axiocam digital camera; -PC with Celeron D processor 347 3.06 GHz LGA775 (FSB 533MHz/L2 Cache 512KB), VIA® UniChrome9 integrated Graphics; 1GB DDR2 (667), 160GB 7200RPM Serial ATA, DVD-R/RW 18x Dual Layer, AOC 15  "LCD LM522;  Software Axiovision Rel. 4.6 Image Acquisition and management in Windows; HP Photosmart Inkjet Printer. Axioscope A1: trilocular microscope with transmitted illumination of 50w for light field, illumination reflected with mercury lamp of 50w for fluorescence, with body for 4 modules. AXIOCAM MRc: Color digital camera with sensitivity for all microscopy techniques, 1.4 MB resolution and Fire interface. 02 Computers, 02 printers, miscellaneous furniture, air conditioning. Wireless network.

 

c) Biomolecular spectroscopy laboratory C V Raman: InPhotonics-RP2785/12-YY-5 Raman system, RamanProbe II™ Immersion Raman probe for use up to 200 oC; For laser excitation wavelength of 785 nm; Cable 5 meters; Working distance 7.5 mm (standard). Choose from 5 mm to 10 mm distances at no additional cost. Optical fiber 100/200 μm. Choice of 200/400 μm or 50/100 μm. Laser Cleanlaze™ BRM-785-0.30-100-0.22-SMA: wavelength 785 +/-0.5 nm; Spectral width: 0.3 nm power > 300 mW; Power supply 110 – 240 AC 50/60 Hz; Terminal connection type FC/APC 105 mm Raman probe for excitation at 785 NM (BAC100-785; BAC100 Raman probe adapter; Raman BAC 150 probe fixer;  AndoriDus™ Camera DU401A-BR-DD, Andor Shamrock™ SR-303I-A, Software Solis™ spectroscopy Software, SR-ASM-8010. Connection, XY adjustable position for optical fiber., SR3-GRT-0300-1000 SR303 GRATG 300-1000 diffraction Grade 300 lines per mm 1100 nm, SR3-GRT-0600-1000 SR303 GRATG 600-1000 diffraction Grade 600 lines per mm 1000 nm, SR3-GRT-1800-DH SR303 H1800/ 300-1100 holographic diffraction grid 1800 lines per millimeter 300 nm at 1000 nm. 01 PC, 01 printer, miscellaneous furniture, air conditioning.

 

d) Microbiology laboratory Robert Hooke: Laminar Flow Chamber Labconcopurifier II class II total exhaust.; Kiln drying and sterilization and drying Fanen model 315SE, greenhouse bacteriological mod JP101, Prolab, various glassware, miscellaneous furniture, air conditioning, 01 PC, 01 printer, wireless network.

 

e) Laboratory of Cell Culture and Cellular Biology: Inverted microscope binoc., chromatic flat optics for light field and phase contrast; CO2 incubator with air jacket 212 liters to 20% CO2, 95% moisture, Filtracão, disinfection by UV, 220 volts; Refrigerated microprocessed Bench Centrifuge-20 ° C to 40 ° C, adjustable speed from 300rpm to 15000 rpm and maximum capacity to 1000ml; Vertical Autoclave 30 Liters 30 X 45 X 115cm; Bath-Maria Serology 45 tubes, capacity for 5 liters, temperature up to 60 ° C, Digital Bench meter pH/MV/Temperature 0-14.00 pH of 0 a100 °c, with combined electrode solutions and buffers. 01 PC, 01 printer, miscellaneous furniture, various glassware, air conditioning. Wireless Network. This laboratory had its capacity expanded by project under the responsibility of its coordinator Prof. Antônio Pinheiro, with resources from CAPES (Edital Infraestrutura 2012) for the acquisition, together with the PG program in biotechnology, of the most of a RAMAN spectroscope.

 

Laboratory of Allergology

 

Also located in the 2nd. ICS Floor has 2 rooms of 60 m² for tests with countertops for 12 people with various equipments; 1 Room of 5 m² of cell cultivation with two streams, 1 room of 5 m2 with CO2 incubator and microscope invertido1 room of 10 m² of mite cultivation 1 room of 10 m²-student office; 1 Room of 20 m². -Teacher's office. This laboratory has the following equipment: ventilated shelving with capacity to shelter both normal and immunodeficient animals; Mechanical Crushers, Western-blot apparatus, ELISA Reader, microtitration washer, 2 CO2 incubators 5 refrigerators, 6 freezers-20, 2 freezers 70 º C, Maria bath, 2 high rotation refrigerated centrifuges, micro centrifuge, Rotational evaporation with thermostatic bath and vacuum pump, 3 cylinders of CO2, liquid nitrogen canister, ThermoCycler, ice maker, hydrophobic interaction chromatography system, Western blot apparatus, 4 laminar flow enclosures, 3  Bacteriological Microscopes, 2 esterioscopes, 1 inverted microscope, photodocumenter, shaker Incubator, bacteriological Greenhouse, 3 BOD greenhouses, drying kiln, autoclave, exhaust chamber, air disdrier, 3 scales.

 

Laboratory of Optical Properties – Lapo, Institute of Physics – UFBA and Multiuser Laboratory of Scanning Electron Microscopy of UFBA.

 

The LaPo incorporated nanotechnology as a tool for obtaining new materials and processes of device developments. The work developed by the team consolidated the laboratory as a unit with infrastructure and know-how for theoretical investigation and characterization of optical, magnetic, conductive and morphological properties. The group also concentrates research in the area of alternative energies directed to the conversion of solar energy into thermal and solar photovoltaic conversion. The group owns three invention patents. The laboratory has a physical area of 200m. Spectrometers for the near-visible ultraviolet-near-infrared range (0.3 to 2.5 µm), functioning in reflection, transmission, absorption (photoacoustics) and photoconductivity (developed in the laboratory). Lockin amplifiers, High voltage sources and photomultiplier tubes, silicon detectors, Germanium, PbS, Pbseepyroelectric, light sensor characterization workbench and photovoltaic cells. Air + air cooled Laser and Lockin amplifier. Controlled power and voltage sources and digital multimeters. Helium cryostat gaseous up to 10K. Sample preparation Bench equipped with Laminar flow chapel. Muffle-like furnace. Sun-gel and electrolytic deposition devices.

The program has computer rooms and support cores in different research laboratories where it is possible to find computers with access to World Network, several indexers and to CAPES journals. The program also provides remote access (VPN) to search and retrieval systems of articles from the library/UFBA.

Through the link http://www.pergamum.bib.ufba.br/pergamum/biblioteca/index.php it is possible to consult online the availability of copies of interest titles.

The academic production developed at UFBA is disseminated through the Internet on a page called Repositório Institucional (Institutional Repository) (https://repositorio.ufba.br/ri/). On this page you can see several publications, which can be searched through the title or unit. It is also possible to access all journals of UFBA in portal periodicals UFBA (http://www.portalseer.ufba.br/).

 

Library

 

The Sectorial libraries were unified by library system of Federal University of Bahia (SIBI), supplementary organ (created by resolution 03/09 of the University Council), responsible for the systemic functioning of the libraries of UFBA, in order To support the development of research, teaching and extension (http://www.sibi.ufba.br).

The SIBI collection is fully computerized, allowing access to the information centers of Brazil and the world. The UFBA has policies to update the bibliographic collection to obtain a good level of performance. Basic Guidelines summarised: